Somatic Cell Nuclear Transfer (SCNT) is a cloning method that involves transferring a nucleus from a somatic cell of the individual to be cloned to an enucleated egg. This activity simulates, step-by-step, the SCNT process used by researchers at the University of Hawaii.

Prior Knowledge Needed

At least a basic knowledge of cell structure and the function of the nucleus and cytoplasm. The nucleus contains the information (DNA, in the form of genes on chromosomes) related to inheritance of traits. The cytoplasm contains the machinery (organelles, proteins, etc.) for the cell to carry out its functions. Artificial embryo twinning is a technique in which a very early embryo is manually separated into individual cells, each of which develops into a "twin."

Time Required

Prep Time: 30 minutes (copying and review time)
Class Time: 45 - 70 minutes


Let's Clone A Mouse, Mouse, Mouse ... Print-and-Go


Supported by a Science Education Partnership Award (SEPA) [No. 1 R25 RR16291-01] from the National Center for Research Resources, a component of the National Institutes of Health, Department of Health and Human Services. The contents provided here are solely the responsibility of the authors and do not necessarily represent the official views of NCRR or NIH.